• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    The hemolytic method for the continuous determination of antistreptolysin O antibodies (ASO) in blood samples consists of reacting a first reagent containing a single dose of oxidised SO with the specific antibodies which may be present in the blood sample under examination, allowing the necessary time to pass for the reaction between the oxidised SO and said antibodies to take place, returning the oxidised SO to its reduced state by adding a second reagent, and measuring the rate of hemolysis. The continuous determination of the ASO titre is obtained by comparing said rate of hemolysis with the rate of hemolysis shown graphically for samples of known ASO titre.
    公开号:SU1367838A3
    申请号:SU813304334
    申请日:1981-07-01
    公开日:1988-01-15
    发明作者:Риччи Антонио
    申请人:Диессе Диагностика Сенесе С.П.А. (Фирма)；
    IPC主号:
    专利说明:

    00 O)

    00
    with
    00
     s
    The invention relates to the field of medicine, mainly to the diagnosis of infectious diseases, and can be used to detect antibodies to hemolytic streptococcus.
    The aim of the invention is to simplify and increase the accuracy of the determination.
    The method is explained in the following examples.
    Example 1, Determination of anti-streptolysin-0 antibodies in whole blood,
    To 0.01 ml of whole blood, taken for the study, add 2 ml of a buffered physiological solution of 0.15 M at pH 7.0 and 20 ° С containing 5 s V / MP of oxidized streptolysin. stirring thoroughly, the mixture is left at room temperature for 15 minutes, after which 0.1 ml of a dithioerythritol-reducing solution is added to the reaction mixture (dithioet etol, mercaptoethanol or their mixture at a concentration of 0.001 mol / l, the reaction mixture is stirred and measured its optical density at 600 nm. Analysis of the spectra obtained with this The photophotographic curve showed that the change in the spectral absorption of the reaction mixture was 21.5 divisions / min. This indicates that the blood sample under study has an antistreptolysin titer — 0-ovid antibody 430 IU. At the same time, the determination by a known method gave equal to 333 ME,
    PRI me R 2 Definition of anti-streptolysin-0 antibodies in serum, I
    The solution of oxidized streptolysin-0 was used at a concentration of 5 s V / ml, and erythrocytes were previously added to this solution (0.1 ml of erythrocyte sludge for every 40 ml of the solution of oxidized streptolysin-0 in physiological solution), human erythrocytes of group O, Rh (rabbit erythrocytes can be used). To determine the antibody titer of 0.01 MP of the serum tested, a 4 MP solution of oxidized streptolysin-0 containing the erythrocyte suspension is added to the mixture and the mixture is thoroughly mixed .. Then the mixture remains
    for 15 minutes at room temperature, after which 0.2 ml of a reducing solution is added,
    containing dithioerythritol, as described in Example 1, it was found that the rate of change of the spectral absorption was 28.5 m / min. This corresponds to the antibody titer 260 ME, -,
    The proposed method, in comparison with the known method, provides a kinetic determination of antistreptolysin-0 antibodies in the process of hemolysis using oxidized streptolysin, which provides greater measurement accuracy.
    权利要求:
    Claims (1)
    [1]
    Invention Formula
    0
    five
    five
    0
    f. The method for determining antistreptolysin-0-ovg-antibodies in blood by introducing oxidized streptolysin-0 into the test sample, incubating and determining hemolysis, characterized in that, in order to simplify and improve the accuracy of determination, the lyophilized oxidative streptolysin- 0 in an isotonic buffer solution at a pH of 7.0 at a concentration of 4-8 with V / ml and after incubating the mixture, a reducing agent is introduced into it, containing in its composition a thiol group and in an isotonic solution buffered at g pH 7.0 at a concentration of 0.00 1 mol / l, after which the rate of hemolysis is measured, and the titer of antistreptosisin 0 antibodies in the test sample is determined using a calibration graph constructed for samples with a known content of antibodies
    2, a method for determining antistreptolysin-0 antibodies in blood by 5 introducing an oxidized streptolysin-0 into a test sample, incubating and determining the degree of hemolysis, characterized in that, with a chain of simplification and increase in the accuracy of determination, blood serum is used as the test sample in which lyophilized oxidized streptolysin-0 is introduced in isotonic buffered at pH 7.0 solution at a concentration of 4-8 with V / ml and after incubating the mixture, an additional step is performed to add red blood cells in the amount of equivalent to whole blood, after che0
    0
    iZ13678384
    A reducing agent, antistreptolysin-0-antibodies, are introduced into the composed thiol group sample, which is in an isotonic order. 3. The method according to claims 1 and 2, OTL and - buffered at pH 7 , 0 to a solution, whereby a hemolysis rate is used as a 0.001 mol / L concentration and a hemolysis rate is used, followed by a dithioerythritol, or mercaptoethanol, using a calibration graph, post- or dithiothreitol in a concentration of x-ray for samples with known. 0.001 mol / l, or a mixture of these agents is contained antibodies, determine the titer with a final concentration of the mixture
    0.001 mol / l.
    类似技术:
    公开号 | 公开日 | 专利标题
    US4302536A|1981-11-24|Colorimetric immunoassay process
    Pembrey et al.1972|Reliable routine estimation of small amounts of foetal haemoglobin by alkali denaturation.
    Rosano et al.1977|A radioimmunoassay for human serum myoglobin: method development and normal values.
    US4804625A|1989-02-14|Assay procedures
    EP0005978A1|1979-12-12|Process for detecting and quantifying an antigen or antibody
    Schosinsky et al.1987|Simple spectrophotometric determination of urinary albumin by dye-binding with use of bromphenol blue.
    US3957436A|1976-05-18|Resultant color step indicator
    SU1367838A3|1988-01-15|Versions of method of determining tistreptolizene antibodies in blood
    Van Lente et al.1979|Evaluation of a nephelometric assay for haptoglobin and its clinical usefulness.
    US3558278A|1971-01-26|Determination of albumin
    US4714672A|1987-12-22|Immunoassays involving complement lysing of chromophore containing microcapsules
    Buffone et al.1979|Limitations of immunochemical measurement of ceruloplasmin.
    EP2309265A1|2011-04-13|Method of assaying complex and kit to be used therefor
    US4321058A|1982-03-23|Latex composition and method for determining erythropoietin
    RU2203499C1|2003-04-27|Method for carrying out diagnostic reaction at brucellosis
    Shukla et al.1974|Quantitation of hemoglobins by immunodiffusion: Specific antibodies to hemoglobins A1, S and F
    US4554248A|1985-11-19|Composition and method for detecting Antistreptolysin O
    JP3365440B2|2003-01-14|Specific antibody measurement method and measurement reagent
    Martinez et al.1987|Preliminary clinical studies of C-reactive protein quantified by enzyme-linked immunoassay.
    US4399228A|1983-08-16|Polate competitive protein binding assay
    US3733398A|1973-05-15|Determining and reversing anticomplementary activity in complement fixation test for australia antigen
    Yoshikazu et al.1984|A simple and rapid microdiffusion method for blood ammonia using a reflectance meter and a reagent plate, and its clinical evaluation for liver diseases
    CN112129949A|2020-12-25|Retinol binding protein detection kit, preparation method and use method thereof
    JPH0650313B2|1994-06-29|Antigen measurement method
    RU2180117C1|2002-02-27|Method of estimation of sensitization to chemical compounds
    同族专利:
    公开号 | 公开日
    JPS5794659A|1982-06-12|
    DE3172219D1|1985-10-17|
    US4379850A|1983-04-12|
    CA1162846A|1984-02-28|
    IT8023215D0|1980-07-03|
    EP0043608B1|1985-09-11|
    CS252455B2|1987-09-17|
    IT1132167B|1986-06-25|
    EP0043608A1|1982-01-13|
    AT15546T|1985-09-15|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    DE1914081A1|1969-03-20|1970-10-01|Behringwerke Ag|Antistreptolysin reagent for diagnosis in - rheumatic cases|
    TR19262A|1975-12-22|1978-09-19|Sclavo Inst Sieroterapeut|TITRATION PROCEDURE MADE BY THE DEVICE AND THE ELEMENTS OF THIS DEVICE FOR TITRATION OF ANTISTREPTOLINE ANTIBODIES|
    FR2435020B1|1978-08-29|1983-11-25|Suovaniemi Finnpipette|IT1218273B|1981-05-07|1990-04-12|Sclavo Inst Sieroterapeut|INTEGRATED METHOD FOR ANALYSIS OF ANTI-STREPTOLYSINIC ANTIBODIES AND MEDIA SUITABLE FOR THE PURPOSE|
    DE3210080A1|1982-03-19|1983-09-22|Behringwerke Ag, 3550 Marburg|ANTI-STREPTOLYSIN O-LATEX REAGENT AND METHOD FOR THE PRODUCTION THEREOF|
    US4554248A|1982-11-08|1985-11-19|American Home Products Corporation|Composition and method for detecting Antistreptolysin O|
    ES2037045T3|1986-09-24|1993-06-16|Wako Pure Chemical Industries, Ltd.|NEW METHOD FOR DETERMINING THE VALUE OF ANTISTREPTOLISIN AND A CASE USED FOR IT.|
    US5354846A|1988-11-18|1994-10-11|Michael Kehoe|Streptolysin O antigen derivatives, its production and uses|
    WO1999036778A1|1998-01-20|1999-07-22|University Of Victoria Innovation & Development Corporation|Detection of gpi anchored proteins|
    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    IT23215/80A|IT1132167B|1980-07-03|1980-07-03|HEMOLYTIC METHOD FOR THE CONTINUOUS DETERMINATION OF ANTI-STREPTOLYSINE ANTIBODIES OR, IN BLOOD OR SERUM SAMPLES WITH THE USE OF STREPTOLYSINE OR OXIDIZED|
    [返回顶部]